CULTURE METHOD AND CULTURE VESSEL
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United States of America Patent
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Aug 18, 2022
app pub date -
Jul 21, 2020
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Jul 22, 2019
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A culture method comprises: filling a first well 1 and a second well 2 in a culture vessel with a culture medium for neurons, the first well 1 and the second well 2 communicating with each other through a flow channel 3; seeding the first well 1 with neurons N; culturing axons a of the neurons N until the axons a of the neurons N reach the second well 2 through the flow channel 3 and also block the flow channel 3; and thereafter removing the culture medium in the second well 2, filling the second well 2 with a culture medium for cells to be co-cultured with the neurons N, seeding cells C to be co-cultured in the second well 2, and culturing the cells C together with the axons a of the neurons N.
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