DIGITAL MICROFLUIDIC AGGLUTINATION ASSAYS
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United States of America
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Jun 30, 2022
app pub date -
May 4, 2020
filing date -
May 4, 2020
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Abstract
The present disclosure provides a method for performing agglutination assays on a “two plate” DMF device format. Droplets containing analytes of interest (particles, cells, etc.) are loaded into the DMF device and mixed with solution-phase or dried agglutinating antibodies or antigens. The agglutinating agents bind to their complementary targets (e.g. antibodies or antigens for example) in the sample droplets, which leads to the formation of insoluble aggregates. Active mixing on a DMF device reduces the reaction time and enhances the agglutination effect. Since the agglutinated sample is sandwiched between two plates on the DMF device, it is straightforward to visualize the result by eye or via a digital camera.
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