METHODS OF OBTAINING LIPIDS FROM A MICROBIAL CELL COMPOSITION BY ENZYME AND PH SHOCK
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United States of America Patent
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May 19, 2022
app pub date -
Mar 13, 2020
filing date -
Mar 13, 2020
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Abstract
The present invention is directed to a process for obtaining a lipid from a cell, the process involving lysing a cell to form a lysed cell composition; optionally adding a salt to the lysed cell composition; sequentially, in one or more cycles, raising the pH of the lysed cell composition to 10 or above for a period of time, followed by lowering the pH of the lysed cell composition to a pH of less than 6 for a period of time to demulsify the cell composition; and separating a lipid from the demulsified cell composition.
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