METHODS OF OBTAINING LIPIDS FROM A MICROBIAL CELL COMPOSITION
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United States of America Patent
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May 12, 2022
app pub date -
Mar 13, 2020
filing date -
Mar 13, 2020
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Abstract
The present invention is directed to a process for obtaining a lipid from a composition comprising microbial cells, the process comprising: treating the composition comprising microbial cells to a temperature of about 60° C. to about 95° C. at a pH of about 9 to about 11; separating the treated microbial cells into a light phase and a heavy phase; sequentially, in one or more cycles, raising the pH of the concentrated microbial cell composition to 10 or above, followed by lowering the pH of the concentrated microbial cell composition to a pH of less than 6 to lyse the concentrated microbial cell composition, wherein the raising and lowering of the pH also demulsifies the concentrated microbial cell composition; separating the lipid from the demulsified cell composition, and recovering the lipid.
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