GENE EXPRESSION CASSETTE FOR EXPRESSING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST AND METHOD FOR PRODUCING N-TERMINAL METHIONINE-TRUNCATED PROTEIN OF INTEREST BY USING SAME
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United States of America
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Apr 21, 2022
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Dec 26, 2019
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Dec 26, 2019
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Disclosed are a gene expression cassette for expressing an N-terminal-methionine-truncated target protein comprising a nucleic acid encoding a target protein and a nucleic acid encoding a cysteine protease, in which a cysteine protease recognition sequence is inserted between methionine (Met), which is the first amino acid at the N-terminus of the target protein, and the second amino acid and N-terminal methionine is cleaved with the cysteine protease, and a method of producing an N-terminal-methionine-truncated target protein using the same.
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