AMPLIFICATION AND ANALYSIS OF SELECTED TARGETS ON SOLID SUPPORTS
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United States of America Patent
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Issued Date -
Feb 10, 2022
app pub date -
Jan 19, 2021
filing date -
Jan 19, 2021
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Abstract
Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.
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