SIMULTANEOUS TOP-DOWN AND ROTATIONAL SIDE-VIEW FLUORESCENCE IMAGER FOR EXCISED TISSUE
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United States of America Patent
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Issued Date -
Nov 4, 2021
app pub date -
May 1, 2020
filing date -
May 1, 2020
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Abstract
An imager for simultaneously taking full color pictures and fluorescence images from the same perspectives, top-down and side views, is presented that uses multiple cameras looking at the same stage through a beamsplitter and light sources for each view. The imager is configured to work with a particular fluorophore, or at least a predetermined fluorescence excitation wavelength and emission wavelength. A broadband white light source projects through a shortpass or other filter with a cut-off wavelength either lower than or between the excitation and emission wavelengths. Another shortpass or other filter is in front of a color camera with a cut-off wavelength below that of the emission wavelength, while a monochrome fluorescence camera may or may not have additional filters to bring out the fluorescence. These filters may be built into a dichroic mirror of the beamsplitter. In addition, digital processing may boost frequencies in the color image that were dampened by the filters.
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