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Abstract

The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of:

(1) preparing a cell population containing corneal epithelial cells;(2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and(3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an α3 chain, a laminin having an α4 chain and a laminin having an α5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.

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First Claim