WHOLE-CELL BIOCATALYSIS METHOD FOR PRODUCING ALPHA, OMEGA-DICARBOXYLIC ACIDS AND USE THEREOF
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United States of America Patent
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Oct 21, 2021
app pub date -
Jan 26, 2021
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Mar 3, 2020
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The present disclosure belongs to the technical field of biocatalysis and biotransformation, and particularly relates to whole-cell biocatalysis method for producing α, ω-dicarboxylic acids and use thereof. The biosynthetic pathway designed in the present disclosure is divided into three modules to co-express several different enzymes in host cells respectively, and then the whole-cells are used to catalyze the production of α, ω-dicarboxylic acid from cycloalkanes, cycloalkanol and lactones in a cascade reaction. Compared with the chemical method, this process does not produce any harmful gases during the production process, does not require high temperature, high pressure, and complex metal catalysts, and is a green and environmental protection production method.
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