Homologous Recombination Reporter Construct and Uses Thereof
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United States of America Patent
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Sep 9, 2021
app pub date -
Aug 6, 2019
filing date -
Aug 6, 2018
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Abstract
The present disclosure provides homologous recombination reporter nucleic acid construct reagents for increasing the likelihood of detecting successful modification of a specific sequence in chromosomal DNA of a host cell via homologous recombination. The homologous recombination reporter constructs contain a sequence element inserted within the coding sequence for a reporter gene resulting in a mutated reporter gene. The sequence element is removed via homologous recombination based on the presence of two homology regions present in the reporter construct.
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