DEEP LEARNING ENABLED SPATIAL OPTICAL BARCODES FOR POOLED LIBRARY SCREENS
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May 6, 2021
app pub date -
Nov 4, 2020
filing date -
Nov 4, 2019
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Disclosed herein include systems, methods, and compositions for determining perturbations in single cells or performing integrated measurements using, for example, plasmids each comprising (i) a perturbation gRNA with a guide region targeting a chromosome sequence and (ii) barcode gRNAs each with a guide region targeting predetermined spatial region of the genome and zero, one, or more optical detection probe binding sites.
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