ACETYL-COA-DERIVED BIOSYNTHESIS
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United States of America Patent
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Mar 25, 2021
app pub date -
Feb 26, 2019
filing date -
Feb 26, 2018
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Abstract
A method to improve the production of acetyl-CoA-derived biochemicals by overexpression of an acetyl-coenzyme A synthetase or acetate-CoA ligase from naturally acetate-utilizing organisms with or without an added acetate transporter. The production of free fatty acid and its derivatives from renewable carbon source was used as a non-limiting example. Using this approach, the production of free fatty acids with yield close to the maximum theoretical yield at high titer can be achieved. As such, this invention will provide the necessary framework to produce many other products sharing or branching out from the fatty acid synthesis pathway economically. These products include hydrocarbons, fatty alcohols, hydroxy fatty acids, dicarboxylic acids, fatty acid esters, etc.
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