Nanofiber-based long-term primary hepatocyte three-dimensional culture system and culturing method
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United States of America Patent
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Jun 27, 2023
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Jul 30, 2020
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Sep 21, 2018
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Sep 25, 2017
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Abstract
Disclosed is a nanofiber-based long-term primary hepatocyte culture system and a culture method, wherein the primary hepatocyte culture system has an advantage that it can culture cells in three-dimensions in vitro to maintain the original physiological activity of low proliferative primary hepatocytes for a long time by co-culturing indirectly by separating primary hepatocytes and hepatic non-parenchymal cells with a support consisting of nanofibers therebetween without direct co-culture.
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