COMPOSITIONS AND METHODS FOR REPROGRAMMING CELLS AND FOR SOMATIC CELL NUCLEAR TRANSFER USING DUXC EXPRESSION
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United States of America
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Mar 19, 2020
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Oct 19, 2017
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Oct 19, 2017
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Abstract
It was found that DUXC family proteins were efficient activators of EGA and that DUXC proteins could be used in methods in the reprogramming of cells to a totipotent state and to increase the efficiency of somatic cell nuclear transfer (SCNT). Accordingly, aspects of the disclosure relate to a method for reprogramming a cell into a totipotent state, the method comprising expressing a DUXC family protein in the cell. Further aspects of the disclosure relate to a method for making a host cell nuclear transfer (SCNT) embryo comprising expressing a DUXC protein in a somatic cell and transferring the nucleus of the somatic cell to an enucleated oocyte, thereby making a SCNT embryo.
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