Methods For Single-Molecule Fluorescence Amplification Of RNA
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United States of America
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May 31, 2019
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May 31, 2019
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Abstract
The present invention provides novel methods for exponential amplification of labeled nucleic acids with high sensitivity and specificity. In one aspect, the invention includes a method for exponentially amplifying the signal of a fluorescently labeled primary click-amplifying FISH (clampFISH) probe. In another aspect, the invention includes a method for labeling a target nucleic acid in a sample. In yet another aspect, the invention includes a method for detecting a fluorescently labeled target nucleic acid in a sample. The present invention also provides a kit for use with the methods of the invention.
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