FLUORESCENCE-BASED REPORTERS FOR MUTAGENESIS DETECTION IN E. COLI

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United States of America Patent

APP PUB NO 20190330676A1
SERIAL NO

16473442

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Direct detection of mutagenesis in prokaryotes by reversion of an inactivating mutation (reversion mutation assay), producing a quantitative signal for in vivo mutagenesis, may greatly reduce the amount of test chemicals and labor involved in these assays. Further, transcriptional coupling of β-lactamase reversion and GFP, translational fusion between β-lactamase and GFP with stop codon in GFP, and a novel dual reporter to monitor continuous mutagenesis may be used in methods described herein.

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Patent Owner(s)

Patent OwnerAddress
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA1111 FRANKLIN STREET TWELFTH FLOOR OAKLAND CA 94607

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
ALLEN, Jennifer Santa Cruz, US 10 137
CAMPS, Manel Santa Cruz, US 6 5

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