POLYPEPTIDES HAVING HYDROLYTIC ACTIVITY ON 1-KESTOSE IN THE PRESENCE OF SUCROSE BUT LACKING SUCRASE (INVERTASE) ACTIVITY, POLYNUCLEOTIDES ENCODING SAME AND METHODS OF PRODUCTING AND USING SAME IN INDUSTRIAL SUCROSE PRODUCTION FROM 1-KESTOSE
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United States of America Patent
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Sep 12, 2019
app pub date -
Nov 11, 2016
filing date -
Nov 12, 2015
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Abstract
An industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar) by providing a method for the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3 g/L), and comprising the enzymatic hydrolysis (preferably using 1-FEH enzymes—EC 3.2.1.153) of 1-kestose. The process further provides a method of producing a polypeptide having 1-kestose hydrolase activity and a composition comprising the polypeptide.
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