METHOD FOR PRODUCING NEURAL STEM CELLS, MEDIUM, SUPPLEMENT, SUPPLEMENT SET, MEDIUM KIT, AND CELL CULTURE DEVICE
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United States of America Patent
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Jul 18, 2019
app pub date -
May 26, 2017
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May 26, 2017
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Abstract
The present invention provides a method for producing neural stem cells, the method including a culture step of culturing cells using a medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that does not contain a TGF-β receptor inhibitor and a protein kinase C inhibitor, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that contains ascorbic acid or an ascorbic acid derivative, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step.
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