METHODS AND COMPOSITIONS FOR PREPARING POLYNUCLEOTIDE LIBRARIES
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United States of America Patent
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May 23, 2019
app pub date -
Nov 14, 2018
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Nov 14, 2018
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Provided herein are methods of producing molar amounts of nucleic acids in a sample by using fixed molar amounts of primers and amplifying nucleic acids in the sample through a sufficient number of rounds to use all the primer. The methods may be used on a plurality of separate samples to produce samples with molar amounts of nucleic acids that are the same, or substantially the same, or that are related in known molar ratios. Further provided herein are normalized, optionally pooled nucleic acid libraries.
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