Importance

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Abstract

[Problem] To provide a novel fluorescent probe for super-resolution imaging that uses fluorescent light emission characteristics that originate from an intermolecular nucleophilic addition-dissociation equilibrium reaction, and to provide a super-resolution fluorescent imaging method that uses the probe. [Solution] A fluorescent probe for super-resolution imaging that comprises a compound represented by formula (I) or a salt thereof [in the formula, X represents an oxygen atom, C(R^a)(R^b), or Si(R^a)(R^b) (wherein R^a and R^b each independently represent a hydrogen atom or an alkyl group), R¹ represents a hydrogen atom or an optionally substituted aryl (provided that, if R¹ is a phenyl group, the benzene ring of the phenyl group does not have a substituent at position 2 or position 6), R² and R³ each independently represent 1-3 identical or differing substituents that are independently selected from the group that consists of hydrogen atoms, hydroxyl groups, halogen atoms, optionally substituted alkyl groups, optionally substituted sulfo groups, optionally substituted carboxyl groups, optionally substituted ester groups, optionally substituted amide groups, and optionally substituted azide groups, R⁴ and R⁵ each independently represent a hydrogen atom or an optionally substituted alkyl group or N(R⁴)(R⁵) forms an amide group or a carbamate group (provided that, if R⁴ or R⁵ is an alkyl group, each may form, together with R², a ring structure that contains the nitrogen atom that is bonded thereto), and R⁶ and R⁷ each independently represent a hydrogen atom or an optionally substituted alkyl group or N(R⁶)(R⁷) forms an amide group or a carbamate group (provided that, if R⁶ or R⁷ is an alkyl group, each may form, together with R³, a ring structure that contains the nitrogen atom that is bonded thereto)], the fluorescent probe for super-resolution imaging being characterized in that the compound represented by formula (I) or the salt thereof undergoes a nucleophilic addition-dissociation equilibrium reaction with a nucleophilic compound that contains an —SH group.

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First Claim