Reducing DNA damage during sample preparation and sequencing using siderophore chelators
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United States of America Patent
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Jul 28, 2020
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Feb 22, 2018
app pub date -
Oct 19, 2017
filing date -
Nov 5, 2014
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Abstract
Embodiments disclosed herein provide reagents and kits for nucleic acid preparation comprising a siderophore. Embodiments disclosed herein provide methods for preparing a nucleic acid library, which comprise: providing a plurality of nucleic acid molecules from a sample; and manipulating the plurality of nucleic acid molecules in a reagent for nucleic acid preparation comprising a siderophore. Further, embodiments disclosed herein provide methods for reducing oxidative damage to a nucleic acid molecule or increasing the Q (phred) score of a sequencing reaction, which methods comprise preparing the nucleic acid molecule in the absence of EDTA.
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