Directional amplification of RNA
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United States of America Patent
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Jul 14, 2020
Grant Date -
Feb 15, 2018
app pub date -
Mar 24, 2016
filing date -
Mar 24, 2015
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Abstract
Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3′ sequence having complementarity to a portion of the RNA molecule, a non-complementary 5′ sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.
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