METHODS FOR GROWING CELLS
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Aug 9, 2017
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Aug 10, 2016
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Abstract
Some embodiments of the invention include methods for growing stem cells comprising growing the stem cells in a cell medium. In certain embodiments, the cell medium has a pH of from about 6.6 to about 7.2. Other embodiments of the invention embodiments include methods for transplanting comprising (a) growing first stem cells in a cell medium having a pH of from about 6.6 to about 7.2, to provide second stem cells and (b) transplanting the second stem cells into a recipient. Other embodiments include the methods where the stem cells that are human stem cells, mouse stem cells, rat stem cells, primate stem cells, or mammalian stem cells. Still other embodiments include growing stem cells in a cell medium having a pH of from about 6.6 to about 7.2 (e.g., from about 6.8 to about 7.0 or about 6.9) that results in modulation of one or more cell properties (e.g., decreased number of cells in the S phase, decreased amount of ROS, etc.) when that property is compared to that of stems cells grown at a pH of about 7.4. Additional embodiments of the invention are also discussed herein.
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