Separation method

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United States of America Patent

PATENT NO 10730908
APP PUB NO 20180044374A1
SERIAL NO

15798784

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The invention relates to a method of isolating an immunoglobulin, comprising the steps of:

    a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml;b) contacting a liquid sample comprising an immunoglobulin with the separation matrix;c) washing the separation matrix with a washing liquid;d) eluting the immunoglobulin from the separation matrix with an elution liquid; ande) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

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Patent Owner(s)

Patent OwnerAddress
CYTIVA BIOPROCESS R&D ABBJORKGATAN 30 UPPSALA 751 84

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ander, Mats Uppsala, SE 38 237
Bjorkman, Tomas Uppsala, SE 41 362
Blom, Hans Uppsala, SE 18 62
Forss, Annika Uppsala, SE 10 182
Hansson, Jesper Uppsala, SE 5 16
Rodrigo, Gustav Uppsala, SE 43 350

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