METHOD FOR CRYOPRESERVING SHEET-SHAPED CELL CULTURE

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United States of America Patent

SERIAL NO

15784460

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Abstract

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Methods are disclosed of freezing, cryopreserving, and transferring a frozen sheet-shaped cell culture. The method of freezing includes (1) a step of immersing in a cryopreservation solution a sheet-shaped cell culture supported by a mesh-shaped support body; (2) a step of removing the cryopreservation solution adhered to the sheet-shaped cell culture, while keeping the sheet-shaped cell culture supported by the mesh-shaped support body; (3) a step of enclosing the sheet-shaped cell culture in a cold-resistant film, an upper surface and a lower surface of the sheet-shaped cell culture being covered by the mesh-shaped support body; and (4) a step of freezing the sheet-shaped cell culture.

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Patent Owner(s)

Patent OwnerAddress
TERUMO KABUSHIKI KAISHA44-1 HATAGAYA 2-CHOME SHIBUYA-KU TOKYO 151-0072
OSAKA UNIVERSITYSUITA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Fukushima, Satsuki Osaka, JP 8 11
Iseoka, Hiroko Ashigarakami-gun, JP 4 6
MIYAGAWA, Shigeru Osaka, JP 50 117
Ohkawara, Hirotatsu Osaka, JP 3 4
Saito, Atsuhiro Osaka, JP 23 121
Sawa, Yoshiki Osaka, JP 83 807

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