Primers, probes and methods for sensitive, specific detection and monitoring of HIV-1 and HCV
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United States of America Patent
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Dec 8, 2020
Grant Date -
Feb 8, 2018
app pub date -
Feb 23, 2016
filing date -
Feb 23, 2015
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Abstract
Primers and probes for detecting an RNA virus, including HIV, HIV-1 subtypes of the M and O groups, and HCV, in a sample. The primers and probes can be used for monitoring the efficacy of anti-retroviral treatment in a subject infected with HIV and/or HCV, and for detecting acute HIV-1 infection, and/or acute HCV infection, in a subject. Included are inner, middle and outer primers that can be used in PCR, including triple nested PCR in a single tube. The methods are highly sensitive and specific, allowing for detection of as few as 4 copies of virus in a sample.
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