Quantification Method for Ammonia, Quantification Reagent, Quantification Reagent Kit, Test Piece, and Ammonia Quantification Device
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United States of America Patent
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Feb 8, 2018
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Aug 2, 2017
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Aug 5, 2016
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A method of quantifying ammonia, the method includes performing a first reaction in which a test liquid containing ammonia is reacted with adenosine triphosphate and L-glutamic acid in the presence of glutamine synthetase to produce phosphoric acid, performing a second reaction in which the produced phosphoric acid is reacted with pyruvic acid in the presence of pyruvate oxidase, and measuring a component consumed or produced by the second reaction, to quantify ammonia, wherein a reaction to produce adenosine triphosphate from adenosine diphosphate mediated by pyruvate kinase is not carried out.
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