Multi-sheath flow and on-chip terminating electrode for microfluidic direct-blotting
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United States of America Patent
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Aug 11, 2020
Grant Date -
Feb 8, 2018
app pub date -
Aug 7, 2017
filing date -
Aug 8, 2016
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Abstract
Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.
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