METHOD FOR DESIGNING PRIMER USED FOR POLYMERASE CHAIN REACTION AND PRIMER SET
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Sep 29, 2017
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Mar 31, 2015
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Provided is a method for designing a primer used for a polymerase chain reaction including: a local alignment step of obtaining a local alignment score by performing pairwise local alignment on a base sequence of a primer candidate under a condition that a partial sequence to be subjected to comparison includes the 3′ terminal of the base sequence of the primer candidate; and a global alignment step of obtaining a global alignment score by performing pairwise global alignment on a base sequence which has a predetermined sequence length and includes the 3′ terminal of the base sequence of the primer candidate and a primer set.
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