NUCLEIC ACID AMPLIFICATION REACTION METHOD, REAGENT SET, AND METHOD OF USING REAGENT SET
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Jul 14, 2017
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Jul 29, 2016
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A nucleic acid amplification reaction method includes a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer, a temperature increasing step of increasing the temperature of the reaction solution to a temperature at which the amplified nucleic acid is denatured after the thermal cycling step, a temperature decreasing step of decreasing the temperature of the reaction solution to a temperature at which a probe hybridizes after the temperature increasing step, and a probe addition step of adding the probe to the reaction solution.
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