NUCLEIC ACID AMPLIFICATION REACTION METHOD, NUCLEIC ACID AMPLIFICATION REACTION REAGENT, AND METHOD OF USING NUCLEIC ACID AMPLIFICATION REACTION REAGENT
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Jul 18, 2017
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Jul 29, 2016
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A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a template nucleic acid, a primer, a probe, and a polymerase, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, the calculated Tm value of the primer is 65° C. or higher and 80° C. or lower, a ΔTm value obtained by subtracting the actually measured Tm value of the primer from the actually measured Tm value of the probe is −11° C. or more and 2° C. or less, the calculated Tm value is a value calculated according to a calculation formula, and the actually measured Tm value is an actually measured value obtained by actual measurement.
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