Materials and methods utilizing biotin producing mutant hosts for the production of 7-carbon chemicals
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United States of America Patent
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Mar 16, 2021
Grant Date -
Jan 25, 2018
app pub date -
Jul 25, 2017
filing date -
Jul 25, 2016
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Abstract
Disclosed are methods for regulating biosynthesis of at least one of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, 7-aminoheptanoland 1,7-heptanediol (C7 building blocks) using a pathway having a pimeloyl-ACP intermediate, the method including the step of downregulating the activity of BioF. Also disclosed are recombinant hosts by fermentation in which the above methods are performed. Further disclosed are recombinant hosts for producing pimeloyl-ACP, the recombinant host including a deletion of a bioF gene.
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