Rapid Detection of Microbial Resistance to Lactam Antibiotics by LC-MS/MS
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Feb 9, 2016
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Feb 11, 2015
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A rapid screening process is provided for identification of bacterial resistance to antibiotics by utilizing LC-MS/MS to quantitate concentrations of parent drugs and also detect hydrolysis products which result from beta-lactamase activity. The susceptibility testing is accomplished in time periods as short as 90 minutes, which includes incubation of bacteria with antibiotics and LC-MS/MS analysis. The antibiotics can be multiplexed for incubation with bacteria to minimize analysis time. 23 different strains of E. coli have been evaluated by this method including ATCC reference (3) as well as clinical isolates (20) and achieved complete concordance with traditional methods. To date the following antibiotics have been tested: penicillin, ampicillin, amoxicillin, cloxacillin, piperacillin/tazobactam, and cefotaxime. All incubations are conducted in the absence and presence of tazobactam which acts as a control. LC-MS/MS analysis was conducted on an AB SCIEX 3200 QTRAP system utilizing positive ion electrospray with MRM detection.
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