Toolkit for the production of post-translationally modified proteins
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United States of America Patent
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Dec 15, 2020
Grant Date -
Jan 11, 2018
app pub date -
Sep 20, 2017
filing date -
Jul 27, 2015
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Abstract
The present disclosure provides a system to produce soluble, folded, and post-translationally modified proteins. The system includes a fusion protein comprising a catalytic domain of an enzyme involved in post-translational protein modification and a targeting domain, and a substrate protein comprising a protein of interest and a sequence that interacts with the targeting domain. The present disclosure also provides polynucleotide sequences encoding fusion proteins and substrate proteins, vectors for expressing polynucleotide sequences, vectors comprising the polynucleotide sequences, and isolated cells comprising said vectors.
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