Importance

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Abstract

The invention relates to a method for determining a biomarker on single cell level by counting a first portion of a cell sample, subjecting said first portion to conditions whereby the biomarker is fragmented, adding to a known number n(k) of labelled biomarker fragments, measuring a first and a second parameter of said first portion, wherein said first parameter corresponds to the amount of said biomarker fragment and said second parameter corresponds to the amount of said labelled biomarker fragment yielding a biomarker fragment value, v(u), and a labelled biomarker fragment value v(k), respectively, and relating v(u) and v(k) with n(k) and c(1), thereby determining an average number of biomarker molecules per cell, m(u), of said first portion. Subsequently, a second portion of the cell sample is contacted with a label specific for the biomarker, a value of the label is determined for the second portion, yielding a single cell measurement value s(u) for the cells of the second portion, a mean measurement value m(s) is determined, and a number of biomarker molecules, n(u) is computed from s(u), m(s) and m(u) for each cell.

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First Claim