METHODS OF MEMBRANE-BASED PROTEOMIC SAMPLE PREPARATION
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Jan 8, 2016
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Jan 9, 2015
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Abstract
A method for rapid isolation of a biological compound (e.g. protein) from an aqueous sample is described herein. The method uses a porous hydrophobic membrane that has an average pore size significantly greater than the size of the biological compound. The method permits the biological compound to attach to the membrane while the aqueous solvent rapidly moves through the membrane under the application of a vacuum. The biological compound that is attached to the membrane can be washed, optionally digested, and eluted for analysis.
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