serotypes O157:H7 and/or O145:H28
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United States of America Patent
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Feb 16, 2021
Grant Date -
Dec 21, 2017
app pub date -
Dec 3, 2015
filing date -
Dec 3, 2014
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Abstract
The present invention relates to methods and kits for the specific detection of Escherichia coli (E. coli) serotypes O157:H7 and/or O145:H28. The methods and kits are based on the detection of newly identified sequence regions, which have a very high sequence identity between E. coli serotypes O157:H7 and O145:H28 and which are not present in any other known E. coli serotype or bacteria. This sequence region thus allows for selective detection of E. coli O157:1-17 and/or O145:H28 from other bacteria, especially other E. coli serotypes. Furthermore the present invention shows that a 3 bp InDel sequence in O157:H7 allows for distinguishing between O157:H7 and O145:H28, which allows for selective detection of O157:H7 over O145:H28 and vice versa. Furthermore, the invention provides oligonucleotides useful for said detection.
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