BUBBLE PRIMERS
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United States of America Patent
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Dec 22, 2014
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Dec 22, 2014
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Abstract
A method for generating sequence ready fragments of nucleotide sequences is described, the method making use of “bubble primers” which include first and third portions which hybridise to a target, and a second partly self-complementary portion which forms an unhybridised loop. The loop contains generic sequences allowing use of sequencing primers. The first portion may be degradable so as to generate an amplicon of sequence of interest flanked by the third portion and the generic sequences of the second portion. In preferred embodiments, the second portion, or the region between the second portion and the third portion, also comprises a tetrad of nucleotides A, C, G, T, allowing calibration of the sequencing reaction.
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