Universal platform for genetic code expansion
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United States of America Patent
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Jul 21, 2020
Grant Date -
Dec 7, 2017
app pub date -
May 31, 2017
filing date -
Jun 3, 2016
priority date (Note) -
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Abstract
Methods and compositions are described for selecting and identifying orthogonal aminoacyl synthetase-tRNA pairs and their use to incorporate unnatural amino acids in a site-specific manner in proteins. Specifically described is a novel E. coli tyrptophanyl synthetase-tRNA pair that functions as both an opal and amber suppressor and that incorporates tryptophan analogs into proteins.
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