Transposase-random priming DNA sample preparation
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United States of America Patent
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Mar 26, 2019
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Nov 30, 2017
app pub date -
May 27, 2016
filing date -
May 27, 2016
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Abstract
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
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