Probes for improved melt discrimination and multiplexing in nucleic acid assays
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United States of America Patent
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Aug 25, 2020
Grant Date -
Nov 9, 2017
app pub date -
Jul 21, 2017
filing date -
Aug 11, 2014
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Abstract
Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.
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