Dynamic switching biosensor
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United States of America Patent
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Apr 14, 2020
Grant Date -
Nov 2, 2017
app pub date -
Nov 12, 2015
filing date -
Nov 12, 2014
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Abstract
An analyte in a matrix is sensed using a sensing device having a detection probe conjugated to a mediator-receptor that is not a binder for the analyte. The sensor device is provided with mediators conjugated to analyte-receptors, where the mediators are selected to bind to the mediator-receptors, and where the analyte-receptors are selected to bind to the analyte. In some embodiments, the mediators are bound to the detection probe by a tether molecule, or tether molecule fragment, or tether domain. In other embodiments, the mediators are not bound to the detection probe. The presence of the analyte is detected by optically or electrically detecting changes of distance between the mediators and the mediator-receptor, indicative of association and/or dissociation events between mediators and mediator-receptor, the characteristics of which are affected by whether the analyte is bound to the analyte-receptor.
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