Method and system for the production of recombinant proteins by cells
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United States of America Patent
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Jun 30, 2020
Grant Date -
Nov 2, 2017
app pub date -
Jul 6, 2015
filing date -
Jul 4, 2014
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Abstract
A cassette sequence for the transformation of a host cell includes at least: a first nucleotide sequence encoding a peptide or protein of interest to be produced by the host cell. The first sequence is linked to a second nucleotide sequence providing resistance to a toxin or encoding an antitoxin peptide to the toxin. The nucleotide sequences are organized in such a way that production of the peptide encoded by the second nucleotide sequence(s) is translationally coupled to production of the peptide encoded by the first nucleotide sequence.
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