PRODUCTION METHOD FOR RETINAL TISSUE
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United States of America Patent
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Issued Date -
Nov 2, 2017
app pub date -
Oct 23, 2015
filing date -
Oct 24, 2014
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Abstract
The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3):
- (1) a first step of culturing human pluripotent stem cells in the absence of feeder cells and in a medium comprising a factor for maintaining undifferentiated state,(2) a second step of culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a Sonic hedgehog signal transduction pathway activating substance to form a cell aggregate, and(3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a 1) a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.
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