Use of caprylic acid precipitation for protein purification
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United States of America Patent
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Aug 11, 2020
Grant Date -
Nov 2, 2017
app pub date -
Nov 3, 2015
filing date -
Nov 3, 2014
priority date (Note) -
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Abstract
In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: a) subjecting the mixture to a first chromatography step; b) recovering the protein of interest in an elution solution; c) adding caprylic acid to the elution solution to form a contaminant precipitate; d) removing the contaminant precipitate from the elution solution; and e) subjecting the post-precipitated elution solution to a second chromatography column, thereby purifying the protein of interest.
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