USE OF TUMOR DISSOCIATION REAGENT IN FLOW CYTOMETRY

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United States of America Patent

APP PUB NO 20170299491A1
SERIAL NO

15486562

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present disclosure relates to a dissociation reagent for tumor tissues. The dissociation reagent does not contain collagenase or trypsin but further contains hyaluronidase or a mixture of hyaluronidase and DNase I. The present disclosure also relates to use of the dissociation reagent in dispersing tumor tissues and detecting expression level of molecular markers on cell surface by flow cytometry. The dissociation reagent of the present disclosure does not cause degradation of molecular markers on cell surface such as CD8, PD-1, Tim-3, Lag-3 and the like, thus does not affect downstream assays.

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Patent Owner(s)

Patent OwnerAddress
WUXI APPTEC (SUZHOU) CO LTD215168 NO 1318 WUZHONG AVENUE WUZHONG ECONOMIC DEVELOPMENT ZONE SUZHOU JIANGSU SUZHOU CITY JIANGSU PROVINCE 215168

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
JI, Qunsheng Suzhou, CN 4 0
LIU, Yan Suzhou, CN 664 5733
ZHANG, Ning Suzhou, CN 524 4883
ZHANG, Qiyao Suzhou, CN 3 6

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