Method for estimating the probe-target affinity of a DNA chip and method for manufacturing a DNA chip
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United States of America Patent
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Mar 30, 2021
Grant Date -
Sep 21, 2017
app pub date -
Nov 30, 2015
filing date -
Dec 1, 2014
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Abstract
A method for estimating the affinity ϕ of a first DNA strand, or “probe”, to be hybridized with a second DNA strand, or “target”, to form a hybrid of length Lbp, the method comprising: in each division of a set of M divisions of the hybrid, counting the number of times in which each hybrid of a set of P DNA strand hybrids is present in the division, the hybrids being of length k less than the length Lbp, or “k-hybrids”; for each combination of mismatches of a set of L combinations of mismatches in a hybrid of length Lbp, determining whether the pair of mismatches is present in the hybrid; and calculating the affinity ϕ according to the relation:
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