Tevi chimeric endonuclease and their preferential cleavage sites
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United States of America Patent
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Aug 11, 2020
Grant Date -
Sep 14, 2017
app pub date -
Mar 22, 2017
filing date -
Feb 1, 2013
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Abstract
The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases I-TevI. More precisely, the invention relates to the deciphering of new preferential I-TevI cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.
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