Methods for Enriching for a Population of RNA Molecules
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Apr 25, 2016
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Dec 5, 2013
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A method of enriching for a population of RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding an affinity tag to the 5′ end of 5′-diphosphorylated or 5′-triphosphorylated RNA molecules in a sample by incubating the sample with an affinity tag-labeled GTP and a capping enzyme; and (b) enriching for RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag.
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