METHOD OF PREPARING OLIGONUCLEOTIDE POOL USING ONE OLIGONUCLEOTIDE
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Sep 7, 2017
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Mar 6, 2017
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Mar 4, 2016
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Disclosed is a method of preparing an oligonucleotide pool having various combinations of base sequences using one oligonucleotide and next-generation sequencing (NGS). The oligonucleotide pool prepared according to the present invention does not require the use of conventional microarray chips which are expensive and time consuming, and improves efficiency in terms of cost and time by reducing the number of synthesis steps performed to obtain various combinations of oligonucleotides. In addition, according to the method of the present invention, the steps of preparing an oligonucleotide pool can be simplified, the number of combinations of oligonucleotides that can be prepared and utilized can be exponentially increased. Therefore, according to the method of the present invention, applications of genetic materials can be broadly extended.
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